雌激素对大鼠肝脏缺血再灌注损伤的影响

Effects of Estrogen on Liver Ischemia Reperfusion Injury in Rats

目的:探讨雌激素对雄性大鼠肝脏缺血再灌注损伤的保护作用及其机制。方法:将60只雄性SD大鼠随机分成3组,每组20只,A组:假手术组;B组:肝脏缺血再灌注组(I/R);C组:肝脏缺血再灌注+雌激素处理组。选取肝脏损伤典型指标:血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(AKP)、肝组织中中性粒细胞(PMNs)浸润量、肝脏组织形态学变化以及血清肿瘤坏死因子-α(TNF-α)等,比较各组间上述指标的变化情况,并进行统计学处理。结果:B组、C组与A组比较,血清ALT、AST、AKP以及血清TNF-α升高,但B组明显高于C组;B组、C组均可见肝脏损伤,但B组明显重于C组。结论:雌激素对肝脏I/R造成的损伤有显著保护作用,其作用机制可能与雌激素降低肝脏I/R时血清TNF-α水平有关。

Objective: To study the mechanism and the effect of estrogen on ischemia-reperfusion(I/R) injury in male rat liver. Methods: Sixty SD male rats were randomly divided into 3 group, A: shame operation group(n=20); B: ischemia-reperfusion group (n= 20);C:estrogen treatment group(n=20). Serum ALT、AST、AKP、tumour necrosis factor-α(TNF-α) and the numbers of polymorphonu-clear cells (PMNs) in liver were analysed statistically among three groups. Results: Group B had higher levels of ALT、AST、AKP and TNF-α than group C. Conclusion: Estrogen significantly protects liver from ischemia-reperfusion injury. The mechanism of protection may be related to decreased serum TNF-α.