老年人胃粘膜胃癌相关抗原MG_7-Ag、MGb_1-Ag及MGd_1-Ag表达状况的免疫组化研究

Tumor-associated antigens MG_7-Ag, MGb_1-Ag and MGD_1-Ag in gastric mucosa of old patients:A immunohistochemical study

应用抗人胃癌单克隆抗体MG_7,MGb_1,MGd_1,抗生物素生-物素-酶复合物(ABC)免疫组化法观察99例60岁以上老年人胃窦部、胃体部及赉门部的胃粘膜肿瘤相关抗原的表达状况。发现MG_7,MGb_1,MGd_1相应抗原在胃癌组织中表达阳性率达85～95％,在肠上皮化生病变部位分别有8.7％,15.2％,28.3％,在不典型增生病变部位有40％、80％、08％。也可发现局灶性弱阳性或阳性反应,而在无肠上皮化生或无不典型增生的慢性表浅性胃炎或慢性萎缩性胃炎则无一例出现阳性反应。本文拟对肿瘤相关抗原表达阳性者进行随访,以期发现早期胃癌。

Through ABC immunohistochemical method,the expressions of tumor- associatedantigens recognized by monoclonal antibodies MG_7, MGb_1 and MGd_1 against gastricearcinoma in mucosa of antrum, fundus and cardia of patients over 60 years of agewere studied.It was found that the percentage of positive staining by MG_7, MGb_1and MGd_1 is 85-95% in gastric cancer tissue, 8.7-28.3% in intestinal metaplasia(IM), and 40-80% in atypioal proliferation (ATP), but the intensity of staining wasweak and the pattern of distribution was focused on IM and ATP. There was no po-sitive reaction in cases of chronic superficial gastritis and chronic atrophic gastri-tis without IM or ATP.The follow-up study is indicated in patients with positivestaining by MG_7, MGb_1 and MGd_1, especially by MG_7, so as to detect gastric car-cinoma in early stage.(Article in page 93)