Toll样受体-4介导诱发型一氧化氮合酶mRNA在鼻黏膜上皮细胞中的表达

Expression of inducible nitric oxide synthase mRNA in epithelial cellof nasal mucosa is upregulated through Toll-like receptor-4

目的 :探讨Toll样受体 4 (TLR 4 )对诱发型一氧化氮合酶 (iNOS )在鼻黏膜上皮细胞中表达的上调作用。方法 :应用核酸分子原位杂交技术检测 30例慢性鼻窦炎患者 (鼻窦炎组 )和 2 1例健康者 (对照组 )鼻黏膜上皮细胞中TLR 4和iNOSmRNA的表达。结果 :TLR 4和iNOSmRNA在鼻窦炎组鼻黏膜上皮细胞中的表达 ,分别为 (0 .14 33± 0 .0 184 0 ) /A和 (0 .136 8± 0 .0 0 76 5 ) /A ,分别与对照组 [(0 .10 2 4± 0 .0 1133) /A和 (0 .0 72 4± 0 .0 136 4 ) /A ]比较 ,差异有统计学意义 (均P <0 .0 1) ,且二者表达呈正相关 (r =0 .4 35 ,P <0 .0 1)。结论 :鼻黏膜上皮细胞通过TLR 4识别病原微生物 ,上调iNOSmRNA的表达 ,增加NO的合成 ,以杀伤、清除病原菌 ,增强宿主防御和免疫应答。

Objective:To:investigate the upregulation of inducible nitric oxide synthase(iNOS) in epithelial cell of nasal mucosa through Toll-like receptor-4(TLR-4) .Method:Specimens from 30 patients of chronic sinusitis and 21 healthy adults were examined by in situ hybridization for TLR-4 and iNOS mRNA.Result:All 30 samples of chronic sinusitis showed a stronger expression of TLR-4 and iNOS than in controls(P<0.01).iNOS upregulated in nasal epithelium correlated with TLR-4 (r=0.435,P<0.01).Conclusion:It suggested that TLR-4 may play a role in enhancing local host defence and inflammation via upregulating the expression of iNOS mRNA and the subsequent increased production of NO.