急性脑梗死患者白细胞粘附分子CD_(11a)、CD_(11b)的表达

EXPRESSIONS OF CD_(11a) AND CD_(11b) ON LEUCOCYTES INTHE PATIENTS WITH ACUTE CEREBRAL INFARCTION

目的:探讨缺血性脑血管病白细胞与内皮细胞粘附增强的机理。方法:采用流式细胞术,用单克隆抗体(McAb)定量测定了42例急性脑梗死患者周围血中白细胞CD11a、CD11b的阳性表达率并与40例健康人作对照。结果:急性脑梗死组血液CD11a在单核细胞、粒细胞、淋巴细胞的阳性表达率均明显高于对照组P<0.05CD11b在粒细胞和单核细胞的阳性表达率也明显高于对照组(P<0.05)但它在淋巴细胞上的表达两组间则无显著性差异。结论:急性脑梗死发生时白细胞CD11a、CD11b表达上调,白细胞与内皮细胞的粘附明显增强可能会加重缺血后迟发性神经元死亡的病理过程。

Objective To investigate the mechanism of adhesion increase between leucocytes and endothelial cells in ischemic cerebrovascular diseases. Method Expressions of CD11a and CD11b were tested in vitro by Flow Cytometry (FCM) for 42 patients with acute cerebral infarction and 40 healthy subjects as the controls. Results The patient group with acute cerebral infarction showed significantly higher rates for expressions of CD11a on monocytes?granulocytes and lymphocytes(P < 0.05) and the CD11b expression in the patient group was also significantly elevated on monocytes and granulocytes (P < 0.05 ) compared with the control group. But the CD11b expression did not show statistical difference on lymphocytes between cerebral infarction and control groups. Conclusion These indicate that the expressions of CD11a and CD11b on leucocytes are up-regulation in cerebral ischemic damage and adhesion between leucocytes and endothelial cells obviously increases, which may aggravate post-ischemic delayed neuronal death. [