亚甲兰对硝酸羟胺中毒小鼠存活率的影响

Effect of methylthionium chloride on survival rate of mice intoxicated by hydroxylamine nitrate

目的 探讨解毒药物亚甲兰对硝酸羟胺 (HAN)中毒小鼠的救治效果 ,为HAN中毒的现场药物救治提供依据。方法 ICR雄性小鼠 ,18～ 2 2g ,HAN不同剂量染毒 ,测定腹腔注射HAN对小鼠的LD50 ,制作HAN急性中毒小鼠模型。染毒后立即腹腔注射亚甲兰 ( 9 0 91mg/kg) ,连续 7d观察小鼠的表现和存活情况。结果 HAN染毒小鼠的LD50 为 14 9 6mg/kg ,在LD60 的染毒剂量下 ,染毒组和亚甲兰治疗组的存活率分别为 5 6 5 %和 10 0 % ,经过统计学分析 ,亚甲兰治疗组的存活率与染毒组的差异有显著性意义。结论 亚甲兰可有效地对抗HAN的毒性。

Objective To investigate therapeutic effect of methylthionium chloride on mice intoxication by hydroxylamine nitrate(HAN).Methods Male ICR mice were intoxicated by different concentration HAN.LD 50 of HAN on mice were determined and acute HAN poisoning mice were made.After intoxication,methylthionium chloride (9 091 mg/kg)were injected into intro-peritoneum of mice.The symptoms and survival rate of mice were observed continually for 7 days after injection.Results LD 50 of HAN on mice was 149 6 mg/kg.At the dosage of LD 60 ,survival rate of methylthionium chloride therapeutic group was 56 5%.Conclusion Methylthionium chloride can antagonize HAN intoxication effectively.