雌激素及其受体在急性肺损伤中作用的研究

Study on the function of estrogen and estrogen receptor in acute lung injury

目的 探讨雌激素 (estrogen ,E)及其α、β两种受体 (ERα、β)在急性肺损伤 (acutelunginjury,ALI)中的作用。 方法 健康雄性Wistar大鼠 4 5只随机分成正常组、损伤组、治疗组 ,每组 15只。观察肺组织病理变化、湿干重比 (W/D)、肺血管通透性变化、ERα和ERβ的表达变化。结果 ①病理变化、W/D、肺血管通透性值提示雌激素对ALI有保护作用。②正常组ERβ表达更高。损伤后ER均有升高 ,以ERβ升高明显。治疗后两者亦升高 ,仍以ERβ升高为主。 结论 ①雌激素对ALI起保护作用并与ERβ升高密切相关。②ERα、ERβ在创伤后均升高 ,且ERβ升高更明显 。

Objective To explore the possible effect of estrogen and estrogen receptor in acute lung injury(ALI). Methods 45 male Wistar rats were chosen and divided into 3 groups randomly: control group(group A), Oleic acid(OA)+endotoxin Group(group B), E2+OA+endotoxin Group(group C). In rats' lung with ALI induced by Escherichia coli lipopolysaccharide, we observed pathological changes after intervention by supplying estradiol, and measured W/D, pulmonary vascular permeability (PVP) and pulmonary alveolar permeability index (PAPI); ERα and ERβ in lung of rats were examined by RT-PCR. Results ①Pathology: the lung of the rats of group A was normal. The injury of the rats of group B was very severe. The injury of the rats of group C was significantly relieved.②W/D, pulmonary vascular permeability (PVP) and pulmonary alveolar permeability index(PAPI): the odds of group B were more than that of group A. The odds of group C was less than that of group B; PVP and PAPI of group B were more than those of group A. Numeric value of group C decreased after intervention by supplying estradiol. ③RT-PCR:ERα and ERβ were all transcripted in three groups. ERβ was significantly higher than ERα in group A. They all increased in group B after injury. There was significant difference in ERβ while ERα was not. ERβ rose continually and significantly in group C while ERα didn't. Conclusions ①Estrogen plays a protective role in the LPS-induced ALI,and the role is correlated with set-up of its receptor,especially ERβ. ②ERα and ERβ all increased after trauma. ERβ rose significantly. It indicated that it may play an important role in immunoprotective after trauma.