大鼠结肠中水通道蛋白4的表达与分布

Expression and distribution of aquaporin 4 protein in the colon of rats

目的 :研究水通道蛋白 4 (AQP4 )在大鼠结肠中的蛋白表达和分布 .方法 :选用成年健康SD大鼠 ,采用免疫组织化学对结肠近段和远段中AQP4的表达进行定位检测 .结果 :结肠全段均有AQP4的表达 ,近段的表达水平高于远段 ;AQP4阳性细胞主要分布在黏膜的顶端 ,以吸收细胞为主 ,杯状细胞无表达 ;AQP4主要位于细胞膜上 ,以基底侧为主 .结论 :AQP4在结肠中的表达及其空间上的分布提示其参与了结肠的水吸收功能 .

AIM: To study the expression and distribution of aquaporin 4 (AQP4) in the colon of Spraque Dawly rats. METHODS: Expressions of AQP4 in rats both proximal and distal colon were performed by immunohistochemistry and a monoclonal antibody against AQP4. RESULTS: AQP4 protein was present throughout the whole colon. Its expression in proximal colon was greater than that in distal colon and its expression was decreasing from the tip toward base of the villus. AQP4 protein was present at the basolateral membrane of the absorptive cells lining the villus tip of the colon. The intercalated goblet cells was not labeled. CONCLUSION: Positive AQP4 in rats colon strongly suggests that this protein function as a pathway for water transport in the epithelium.