Dubin Johnson综合征的肝细胞超微结构观察

Ultrastructural observation of Dubin Johnson syndrome in hepatocyte

采用肝穿刺的方法,利用透射电镜对DubinJohnson综合征(结合型高胆红素血症)患者进行了肝细胞超微结构的电镜观察。观察结果:电镜下可见到肝细胞内及毛细胆管周围有大量的外有单层膜包绕的色素颗粒,颗粒大小不均匀;其内容物有三种形态,即电子密度较高且均匀的颗粒,电子密度略低的颗粒及电子密度不均匀的内含有小脂滴样物质的颗粒;毛细胆管扩张,腔内微绒毛减少,管壁增厚;粗面内质网及滑面内质网明显扩张成许多小囊泡,使得肝细胞成筛孔状,肝细胞间有胶原纤维增生,肝细胞间隙增宽,并有微绒毛出现。其结果有助于临床的诊断。

The ultrastructure of hepatocyte of Dubin Johnson syndrome has been observed under electron microscope by the method of liver puncture. Result: There were many pigment granules with monolayer adventitial coat in hepatocyte and around bile capillary. The granules were asymmetry, which include three conformations: symmetrical granules with high electron density; granules with slightly low electron density; granule with asymmetrical electron density and lipid droplet inside. The bile capillary dilated with decrease of microvilli and thickening of the wall. It was observed that rough endoplasmic reticulum and smooth endoplasmic reticulum dilated into many small vesicles, which made hepatocyte cribriform and led to the hyperplasia of collagen fibre in between the liver cells. This result is useful for the clinic diagnosis.