实验性急性出血坏死性胰腺炎时肝脏的病理形态学观察及肝细胞的凋亡

Pathomorphological observation of liver injury caused by experimental acute hemorrhagic and necrotic pancreatitis and apoptotic cell death of hepatocytes in liver

目的:从实验性急性出血坏死性胰腺炎(AHNP)早期开始动态观察大鼠肝组织的病理形态学变化,研究肝细胞凋亡的发生情况。方法:胰胆管内逆行注射5%的牛磺胆酸钠,建立大鼠急性出血坏死性胰腺炎模型,通过光镜及电镜观察肝组织的病理形态学变化,用原位末端标记法(TUNEL法)测定大鼠肝细胞发生凋亡的情况。结果:诱导AHNP3h后大鼠肝细胞出现气球样变性,随着疾病的发展损伤加重,肝细胞发生坏死,炎性细胞浸润,并有血栓形成;肝细胞在诱导3h后出现凋亡,且随时间延长,凋亡的数目也增多,肝细胞出现凋亡和坏死并存的现象。

Objective: To observe the pathomorphological changes in liver at the early phase of acute hemorrhagic and necrotic pancreatitis (AHNP) and apoptosis of the liver cell. Material and method:AHNP was induced in rats by intraductal administration of 5% sodium taurocholate. The pathologic morphologic changes of lung were observed under light microscope and electron microscope. The development of apoptosis in liver cell was examined by TUNEL(terminal deoxynucleotidyl transferase nick and labeling). Results:Hydropic degeneration could be found in the liver 3h after the inducement.The injury of hepatocyte increased in severity with the development of the disease. Necrosis, inflammatory reaction and micro-thrombus in liver were observed. A few apoptotic hepatocytes were found 3h after inducement. The number of apoptotic hepatocyte tend to increase with the development of the disease. Both necrosis and apoptosis of hepatocyte appeared simultaneously.