脂多糖注入黑质对大鼠黑质纹状体单胺类递质和行为的影响

Effects of intranigral injection of LPS on the levels of monoamine transmitters in the nigrostriatal system and behaviours in rats

目的 探讨脂多糖 (L ipopolysaccharide,L PS)对大鼠行为学和黑质纹状体单胺类递质的影响。方法 采用立体定位注射 5μg L PS入大鼠脑黑质 ,在不同时间点观察大鼠注射阿朴吗啡后的旋转行为学 ,及采用HPL C测定黑质纹状体单胺类递质的含量变化。结果 L PS注射后 14、2 1、3 0 d,大鼠出现向注射侧的旋转行为 ,在黑质和纹状体 ,DA及其代谢产物随时间改变呈不同程度下降 (P<0 .0 5) ,而 5-HT仅有短暂下降 ,NA无变化。结论 L PS注入黑质特异性损害 DA能神经元 ,可降低黑质纹状体 DA及其代谢产物含量 。

ObjectiveTo investigate the effects of i ntranigral injection of lipopolysaccharide (LPS) on the levels of monoamine transmitters in the nigrostr iatal system and behaviours in rats. MethodsLPS was stereotaxically injected into substantia nigra (SN). At different survival times,rotation test induced by apomorphine was performed in all groups after LPS injection. The levels of monoa mine transmitters in the nigrostriatal system were measured by using HPLC-ECD. Results14,21,30days after injection of 5μg LPS into t he rat SN,the rotational behaviors were observed. The levels of DA and its metabolites in the SN and striatum decre ased,while the levels of 5-HT in the SN and striatum transiently changed and the lev els of NA showed no change. ConclusionLPS mainly affects th e dopaminergic system and can reduce the DA and its metabolites in the nigrostri atal. Also LPS can induce the rotational behaviors change in rats.