凋亡基因在缺氧缺血新生大鼠脑组织中的动态变化及银杏叶提取物的保护效应

A study on the change of apoptotic genes and protective effect of EGb in hypoxic ischemic brain damage of neonatal rats

目的 探讨银杏叶提取物 (EGb)对新生大鼠缺氧缺血性脑损伤 (HIBD)的保护作用。方法 夹闭妊娠大鼠子宫血管 ,制成 HIBD新生鼠模型 ,治疗组给予腹腔注射 EGb,在生后不同时间比较两组仔鼠脑组织凋亡基因 Bcl-2、Bax的变化及神经细胞的凋亡情况。结果 缺氧缺血后随再灌注时间的延长 ,脑组织中 Bcl-2与 Bax的比例下降 ,同时凋亡细胞数增加 ,表明 Bcl-2、Bax两者比值的降低促进凋亡的发生。EGb治疗后 Bcl-2表达增加显著 ,同时观察到治疗组的凋亡细胞数量减少。结论 EGb对

Objective To investigate the protective effect of extract of ginkgo biloba(EGb ) on hypoxic ischemic brain damage (HIBD) in neonatal rats. MethodsThe HIBD animal models were made by ligating the uterine arteries of pregnant rat,EGb was injected into the HIB D models. The expression of Bcl-2,Bax protein in brain after HI were observed. ResultsA shift in the ratio of Bcl-2 to Bax may contribute to neuronal apoptosis after HI. Overexpression of B cl-2 protects cell from apoptosis,but Bax may be function as a cell death effector protein. The st udy showed that Immunostaining of Bcl-2 protein was increased while the Bax protein were decreased with the treatment of EGb after HI. EGb decreased the apoptosis of cerebral cells. ConclusionEGb has protective effect on HIBD.