结、直肠癌细胞核酸双参数定量测定的临床意义

CLINICAL SIGNIFICANCE OF TWO PARAMETER CELLULAR NUCLEAR ACID CONTENTS OF COLORECTAL CANCER DETERMINED BY MICRO-SPECTROPHOTOMETRY(MSP)

用显微分光光度木测定了24例结、直肠癌及其对照肠粘膜细胞的DNA和RNA相对含量。异倍体肠癌细胞RNA和DNA含量,均非常明显大于其对照肠粘膜细胞(P<0.01)。与对照肠粘膜细胞相比,二倍体肠癌细胞的DNA含量无显著差别,而RNA含量明显增加(P<0.05),因此DNA/RNA值,二倍体肠癌明显小于其对照肠粘膜(P<0.05)。24例结、直肠癌细胞的RNA与DNA平均含量行相关比较,相关系数(r)=0.90,P<0.01,是非常明显正相关。

The cellular DNA and RNA relative contents in 24 pantients of colorectal cancerand control normal colonic mucosa were determined by MSP. The cellular DNA andRNA contents of tumorous heteroploidy were markedly higher than that of the normalcolonic mucosa(P<0.01). The cellular DNA content of tumorous diploidy had no signi-ficant difference to the normal colonic mucosa, however, the cellular RNA content ofthe tumorous diploidy was markedly higher than that of the normal colonic mucosa (P<0.05). A correlation detection of the cellular RNA and DNA contents showed that apositive correlation existed batween RNA and DNA values of the same coloreetal cancerspecimen,correlation coefficient(r)=0.9,P< 0. 01.