结合RGD肽的聚酯材料表面粘附内皮细胞的抗剪切力研究

Study on Fluid Shear Stress Response of Endothelial Cells Attached on Polyethylene Terephthalate Surfaces Covalently with Synthetic RGD Peptides

精氨酸 甘氨酸 天门冬氨酸 (RGD)是许多粘附蛋白的高度保守氨基酸序列。生物材料表面结合RGD肽有助于内皮细胞在材料上的粘附、迁移和增殖。本研究在体外流动条件下观察结合RGD肽或纤维粘连蛋白的聚酯材料表面粘附内皮细胞的抗剪切能力 ,并通过观察肌动蛋白和踝蛋白的表达初步探讨影响细胞粘附稳定性的机制。结果显示材料表面结合RGD或纤维粘连蛋白可以增加细胞的粘附强度 ,提高抗剪切能力 ;

The Arg-Gly-Asp(RGD) tripeptide is recognized as an active series of many adhesive proteins,and RGD grafted onto biomaterials can effectively increase endothelial cells (ECs) adhesion,migration and proliferation.In this study,polyethylene terephthalate(PET) were divided into three groups:Non-coated PET,fibronectin-coated PET,RGD-covalent PET.The adhesive strengths of ECs on the PET surfaces were compared under flow condition(8^19 dyne/cm+2 for 4h),and the expression of F-actin and talin were detected by laser confocal microscopy to study the shear-resistant mechanism of adherent cells.Results showed ECs retention rates of RGD-grafted and fibronectin-coated group were 43^33% and 40^75% respectively,which were much higher than that of non-coated group(13^73%).Results of laser confocal microscopy showed RGD and fibronectin could promote F-actin rearrangement,stress fiber formation and talin expression,which might be the mechanism of EC shear-resistance.