北京市北郊地区宿主动物感染汉坦病毒分子流行病学研究

Study on the molecular epidemiology of hantaviruse carried by hosts in northern suburb of Beijing

目的 了解北京市北郊地区宿主动物携带肾综合征出血热病毒状况和病毒型别。方法夹夜法捕鼠,计算鼠密度,确定鼠种构成。应用逆转录-聚合酶链反应(RT-PCR)扩增M片段部分序列并测序,并用Clustal X(5.0)和DNAClub软件对序列进行分析。检测结果应用SPSS软件进行统计学分析。结果 共捕获宿主动物414只,褐家鼠为优势鼠种,占74.32％。其次为小家鼠,占22.72％。RT-PCR检测宿主带病毒率,海淀区褐家鼠为13.14％,小家鼠为0,昌平区褐家鼠为17.46％,小家鼠为3.57％。对6份扩增阳性标本进行序列测定并对其核苷酸序列进行分析显示,它们均为汉城型(SEO)汉坦病毒。毒株基于M片段部分序列所构建的系统进化树显示,它们可分为两个小进化分支。所测序列在此片段推导出的氨基酸序列多数与标准株完全相同,少数有1.40％～2.16％的差异。结论 北京市北郊地区汉坦病毒的主要宿主动物是褐家鼠,不同宿主动物携带的汉坦病毒之间的核苷酸序列及其推导的氨基酸序列同源性较高,但也存在一定的差异。这种差异形成的原因有待进一步研究。

Objective To investigate hantanvirus infection of captured rodents in Haidian district and Changping district of Beijing and to type hantavirus using molecular technique. Methods The captured mice were classfied and the density of distribution was calculated. Reverse transcription-polymerase chain reaction (RT-PCR) technique was used to amplify the partial M fragnments of hantaviruse. Several representative positive samples were sequenced and analysed by ClustalX (5.0) and DNAClub software. Results A total of 414 animals were captured, among which Battus norvegicus was the dominant group. In Haidian district, the median infection rates with hantavirus were 13. 14% in Battus norvegicus and 0 in Mus)nusculus Linnaeus. In Changping district, the average infection rates were 17.46% in Battus norvegicus and 3. 57% in Mus musculus Linnaeus. Nucleotide sequences analysis showed that the virus detected al1 belonged to SEO-type. They clustered with Z37 viruse and could be branched into 2 different subclades. Conclution The major hosts of hantaviruse in Haidian and Changping district were Battus norvegicus and the epidemic strains in the two districts of Beijing were genotyped as SEO-type. Nucleotide sequence and deduced amino acid sequence from different rodents were highly homologous, while nucleotide mutation had also been observed. Further studies are required to explore the possible viruse sequence mutation.