影响山羊体外受精的因素

Factors influencing in vitro fertilization in goats

以屠宰山羊卵母细胞为材料研究了公羊个体、附睾不同部位精子、成熟培养和受精时卵丘存在与否、卵丘扩展程度及卵龄对山羊体外受精的影响。结果表明 :1)不同公羊精液在受精、卵裂和桑椹 /囊胚率上都有显著差异 ;2 )附睾尾精子和鲜精的受精、卵裂和桑椹 /囊胚率无显著差异 ,但显著高于附睾体和附睾头精子 ;3)成熟培养 2 4和 2 7h卵母细胞的的桑椹胚 /囊胚率显著高于培养 2 1和 30h卵母细胞 ;4 )卵丘扩展 3和 4级卵母细胞受精和桑椹胚 /囊胚率显著高于扩展 0和 1级卵母细胞 ;5 )成熟培养前机械去卵丘严重影响卵母细胞体外受精和桑椹胚 /囊胚率 ;6 )受精前完全去掉卵丘显著影响桑椹胚

We studied the effect of buck individuality,epididymal origin of spermatozoa,presence and absence of cumulus during in vitro maturation and fertilization and length of maturation culture on in vitro fertilization and embryo development in goats.The results are summarized as follows:1)semen from different bucks produced significantly different rates of fertilization,cleavage and morulae/blastocysts;2)rates of fertilization,cleavage and morulae/blastocysts by spermatozoa from cauda epididymidis were not different significantly from those of ejaculated spermatozoa but were markedly higher than those of spermatozoa from corpus epididymidis,and those of spermatozoa from corpus epididymidis were significantly higher than those of spermatozoa from caput epididymidis;3)there was no significant difference in fertilization rates among oocytes in vitro matured for 24,27 and 30 h,but fertilization rates of oocytes matured in vitro for 21 h declined significantly;rates of morulae/blastocysts in oocytes matured for 21 and 30 h were significantly lower than rates among oocytes matured for 24 and 27 h;4)rates of fertilization and morulae/blastocysts in oocytes with 3 or 4 grade cumulus expansion were significantly higher than those of oocytes with 0 or 1 grade cumulus expansion;5)mechanical removal of cumulus prior to in vitro maturation severely affected in vitro fertilization and embryo development to morulae/blastocysts of goat oocytes;6)complete or partial removal of cumulus prior to in vitro fertilization did not affect fertilization and cleavage of oocytes,but complete removal of the cumulus significantly decreased the rate of morulae/blastocysts.In summary,in vitro maturation and fertilization of goat oocytes were affected by many factors.