脱脂华杂3号菜籽饼粕分离蛋白的制备及其特征(英文)

Isolation and Characterization of Defatted Hua-Za 3 Meal Protein

利用H2O、5%NaCl、70%乙醇及0.4%NaOH提取未变性的脱脂菜籽饼粕(华杂3号)中的各种分离蛋白。清蛋白、球蛋白、醇溶蛋白和谷蛋白的比例为3732329。其中清蛋白和球蛋白为主要分离蛋白。SDS-PAGE电泳显示分离蛋白的多肽肽链的各亚基组成。对氨基酸组成进行分析,结果表明菜籽分离蛋白氨基酸含量丰富,组成平衡,不失为一种可食用的优质植物蛋白。

Classical fractionation protein of the undenatured Hua-Za 3 meal, which has been an increasingly important oilseedcrop in China, resulted in H2O-, 5% NaCl-, 70%EtOH-, and 0.4%NaOH-soluble fractions in ratios of 37:32:3:29 respectively.The major proteins in Hua-Za 3 meal were albumin and globulins. The molecular weight of fractionation was shown by SDS-PAGE. Amino acid profile of isolates indicated high nutrition quality for use in consumers' products.