基于分形特征的模拟失重条件下细胞骨架的图像分析

Fractal Analysis for Cytoskeleton Image Under Simulated Microgravity

细胞骨架的结构特点决定了不易找到量化细胞骨架图像的特征参数。为了描述模拟失重条件下细胞骨架的形态变化 ,以评价药物对失重的细胞学效应的防护效果 ,本文利用双毯法和数盒子数法分别计算细胞骨架微管和微丝图像的分形维数 ,利用图像的复杂度描述细胞骨架在不同环境中的形态变化。计算结果表明在模拟失重条件下细胞骨架图像的复杂度减弱 ,加入药物槲皮素后图像的复杂度略有恢复。利用分形维分析细胞骨架在模拟失重条件下图像的纹理变化 。

Cytoskeletal distortion was hard to describe straightly with some quantities due to the complexity of cytoskeletal structure and the morphological uncertainty. For describing the morphological modification of cytoskeleton under simulated microgravity and estimating the confront effect of drugs on cells under simulated microgravity, this paper focuses on get the characteristic parameter that quantify the morphological change of cytoskeletal images. Fractal analysis was applied here to quantify the morphological variation of the cytoskeleton under simulated microgravity. This paper used the methods of covering blankets and box-dimension estimation to count the image complexity for microtubules and microfilaments. To study the medical effect of quercetin, a kind of flavonoids, which could counterwork to effects of microgravity on cells, cytoskeleton image of cells treated by quercetin under simulated microgravity were also analysed. The image complexity of cytoskeleton was decreased under simulated microgravity, but it was partly inhibited by quercetin. The present results demonstrate that fractal dimension could show the texture change of cytoskeletal image under simulated microgravity, and it would be a promising approach to quantitative analysis of the morphological modification of cytoskeleton in different environments.