人胰腺癌细胞中5-LOX基因的表达及其抑制剂的作用

Expression of 5-LOX gene and its inhibitory effect in human pancreatic cancer cell

目的 :探讨人胰腺癌细胞中 5 LOX的表达及其抑制剂的作用。方法 :应用RT PCR、免疫细胞化学技术和图像分析检测 5 LOX在人胰腺癌细胞Capan 2中的表达水平及抑制剂MK886对 5 LOX基因表达水平的影响。结果 :MK886能有效降低 5 LOXmRNA的表达 ,MK886作用组 5 LOX蛋白低表达或不表达 ,对照组均呈高表达。结论 :5 LOX抑制剂能有效阻止 5 LOXmRNA的翻译功能 。

Objective:To detect the expression of 5-LOX and observe its inhibitory effect in humane pancreatic cancer cell.Methods:5-LOX mRNA and protein expression were analyzed by RT-PCR and immunocytochemical staining as well as images analyzing system,and the effect of MK886 was tested.Results:MK886 effectively down-regulated the expression of 5-LOX mRNA.Immunocytochemical staining and images analysis confirmed that the expression 5-LOX protein in cell treated with MK886 was lower than untreated control cell.Conclusion:Inhibitor of 5-LOX can effectively prevent the target mRNA into protein product,thus block or weaken the expression of 5-LOX.