结肠癌组织凋亡相关基因bax及其抑癌基因p^(16)表达及临床意义

The Expression of bax and p^(16) gene in colon tumor and their clinicopathological significance

目的 :了解结肠癌组织中凋亡相关基因bax及其抑癌基因p16的表达 ,并探讨与临床病理学指标及预后的关系。方法 :应用LSAB法 ,将bax与 p16单克隆抗体标记 6 4例结肠癌组织 ,观察bax与 p16基因的表达情况。结果 :bax与 p16在结肠癌组织中阳性表达率分别为 4 8 4 %和 5 4 6 % ;p16的表达与结肠癌的组织类型 (x2 =13.12 ,P <0 0 5 )及 5年生存率 (x2 =4 .2 3,P <0 0 5 )有关 ;bax的阳性表达率在DukesA和B期为 81 2 % ,在C和D期为 4 2 2 % ,两者差异有统计学意义 (x2 =4 16 ,P <0 0 5 )。结论 :bax与p16基因在结肠癌中普遍表达 。

Objective:To determine the relationship between the expression of p 16 and bax gene in colon carcinoma and their clinicopathological significance and prognosis.Methods:immunohistochemical LSAB method was used to evaluate monoclone antibody of p 16 and bax gene in 64 cases of colon carcinoma.Results:The positive rates of bax and p 16 in colon carcinoma were54.6% and 48.4% respectively,positive rate of p 16was significant difference in survival (x 2=4.23,P<0.05)and between well differentiated and poorly groups of tumor (x 2=13.12,P<0.05).The positive-staining rate of bax was significant different at different Dukes stage(x 2=4.16,P<0.05).Conclusion:The expression rate of p 16 and bax gene was very high in colon carcinoma,and it might have a prognosis implications in patients with colon carcinoma.