鼠疫耶尔森菌活疫苗株的比较基因组学研究

DNA microarray-based comparative genomic analysis of live plague vaccines

目的 揭示不同来源的鼠疫活疫苗株在基因组组成上的差异。方法 以芯片比较基因组杂交为主要研究手段 ,结合PCR验证 ,对 19株鼠疫活疫苗株进行比较基因组分析。结果 鼠疫活疫苗株基因组中缺失了大量基因 ,但也有某些大片段基因的拷贝增多。结论 不同来源的疫苗株在基因组组成上存在差异 ,构成了不同疫苗株的表型与使用效果具有差异性的遗传基础。

Objective To identify and compare the genome differences among live plague vaccines prepared with different strains of the bacillus. Methods The whole-genome DNA microarray of Yersinia pestis was used as a tool to perform genomic comparison among live plague vaccines prepared with 19 different strains. Results Dozens of deletions and/or increased copies of the genomic fragments were identified in the studied vaccines of different strains. Conclusion The revealed genomic differences among the vaccines from different origins account for the variability of the immunogenic and protective potency of live plague vaccines. The whole-genome DNA microarray was also provesd to be an ideal tool for the pre-evaluation of a vaccine strain.