利用雄性生殖细胞建立转基因动物

Using the Male Germ Cells to Establish the Transgenic Animal

追溯了用雄性生殖细胞建立转基因动物的发展历程 ,系统阐述了本领域理论和实践的最新进展 ,围绕方法学逐渐改进和完善的过程 ,从利用精子和精原干细胞携带外源DNA两个方向展开 ,分析和评价了DNA转移方法与精子载体法结合、胞浆内单精子注射、蛋白连接的精子介导的基因转移、输精管注射法以及曲细精管显微注射法和精原干细胞移植法 6种实验设计方法。

Tracing back the course of the development in using the male germ cells to establish the transgenic animal and systematically expatiated on the advance of the theories and practice in this field and giving attention to the course of amelioration in methodology,expounding from two directions:one is using the spermatozoa as a vector to carry the exogenous DNA into zygote;the other is using the spermatogonial stem cell to do that.The introduction and assessment about six designs were given,i.e.the combination traditional sperm\|mediated gene transfer with other gene transfecting technique;intracytoplasmic sperm injection;linker based sperm\|mediated gene transfer;transfecting mature sperm in vivo by microinjection into vas deferens;transfecting male stem cells by microinjection into seminiferous tubule and spermatogonial stem cell transplantation.