胸膜肺炎放线杆菌APXⅡA基因的克隆及序列测定

Cloning and sequencing of APXⅡA gene of Actinobacillus pleuropneumoniae

用PCR法从胸膜肺炎放线杆菌 (APP) 7型的DNA提取物中扩增出大小为 2 873bp的APXⅡA基因片段 ;将PCR产物重组到质粒载体 pMD 18 T中 ,并对重组质粒进行酶切分析及序列测定。结果表明 ,克隆片段为完整的目的基因 ,该片段的核苷酸序列与国外分离株M 30 6 0 2的同源性达 99.7%。

The APXⅡA gene of 2 873 bp, which was amplified from Actinobacillus pleuropneumoniae (APP) serotype 7 by PCR, was cloned into the plasmid vector pMD 18-T to construct the recombinant (plasmid). The constructed recombinant plasmid was (analyzed) by restriction enzyme digestion and sequence analysis. The result showed that the cloned gene from APP serotype 7 by us is the full-length target gene and shares 99.7% homology with that from the APP foreign isolate strain M30602.