具有中和活性抗呼吸道合胞病毒单克隆抗体的制备及鉴定

Preparation and Characterization of Anti-Respiratory Syncytial Virus McAb with Neutralization Activity

目的 制备具有中和活性抗呼吸道合胞病毒的单克隆抗体。方法 采用杂交瘤技术制备单克隆抗体 ,并以ELISA结合免疫组化技术进行筛选。结果 获得 8株分泌抗呼吸道合胞病毒的特异性单克隆抗体的细胞株 ,其中 2株分泌的单抗具有中和活性 ,效价 1∶16 0和 1∶32 0。

Objective To prepare anti respiratory syncytical virus McAbs with neutralization activity.Methods Prepare McAb by hybridoma technique,and screen by ELISA combined with immunohistochemical method.Results Eight cell strains secreting McAbs to respiratory syncytial virus were obtainad,of which 2 strains secreted McAb with neutralizing titers of 1∶160 and 1∶320 respectively.Conclusion The anti respiratory syncytical virus McAbs with neutralization activities were successfully prepared.