甘肃省春小麦品种高分子量麦谷蛋白亚基组成分析

Composition of High Molecular Weight Glutenin Subunits of Spring Wheat Varieties in Gansu Province

应用十二烷基硫酸钠 聚丙烯酰胺凝胶电泳 (SDS PAGE)技术 ,对甘肃省 12 1个普通小麦品种 (包括地方品种、育成品种和引进品种 )高分子量谷蛋白亚基构成、不同等位基因间亚基变异及出现频率系统分析的结果表明 ,甘肃省春小麦品种HMW GS等位基因Glu A1编码 1、2 、N (缺失 )三种亚基 ,Glu B1编码 7+ 8、7+ 9、17+ 18、2 2、7、8、14 + 15七种亚基 ,Glu D1编码 2 + 12、2 + 11、5 + 10、2 + 10、10、4 + 11、3+ 12、N八种亚基 ,频率最高的亚基分别是Null( 81 0 % )、7+ 8( 86 0 % )、2 + 12 ( 6 0 0 % ) ;一些优质亚基在现有品种中都存在 ,如甘春 2 0、高台紫麦子等 ,作亲本用于品质改良有很大潜力 ,而且少数罕见亚基 7、8、11也存在 ;不同来源的春小麦品种HMW GS组成比较 ,优质亚基组成类型如 1、7+ 8、5 + 10 ,1、17+ 18、5 +

High molecular weight glutenin subunit (HMW GS) composition of 121 spring wheat varieties including 82 landraces, 11 introduced varieties and 28 modern varieties in Gansu were determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). The results revealed: (1) Glu A1 encoded three subunits of 1,2 * and null, Glu B1 encoded seven subunits of 7+8, 7+9, 17+18, 22, 7, 8,14+15, Glu D1 encoded eight subunits of 2+12, 2+11, 5+10, 2+10, 10, 4+11, 3+12, N. The subunit with the highest frequency is null (81 0%)、7+8(86 0%)、2+12(60 0%)in Glu A1、Glu B1 and Glu D1 respectively; (2) Some good quality subunits existed in studied varieties,such as Ganchun 20、Gaotaizimaizi etc;(4) Compared on HMW GS composition of three source spring wheat varieties, modern varieties had high frequencies of quality subunits Composition 1,7+8,5+10 and 1, 17+18,5+10.