摘要
为了了解菜薹种质内的遗传多样性 ,确定适宜的更新群体 ,从一份菜薹地方品种的 2 0 0株群体中随机抽取 10、15、2 0、2 5、30、35和 4 0株组成不同的群体 ,利用RAPD技术分析各自的多态位点数、计算多态位点百分率、遗传多样性指数和缺失位点数。通过对各参数的比较结果表明 ,该种质内单株间的遗传差异较大 ,大于 30株的群体能代表 2 0 0株群体的遗传多样性。在满足其完全随机交配的前提下 ,可视为更新的有效群体。根据更新时繁种群体应为有效群体 2倍的原则 ,认为不小于6 0株的群体为菜薹种质更新比较适宜的群体。
In order to investigate genetic diversity in populations of Flowering Chinese Cabbage and screen suitable sample size for germplasm regeneration, different bulks with 10,15,20,25,30,35 and 40 plants were formed from an accession of 200 plants of Flowering Chinese Cabbage by randomly sampling. The number of polymorphic loci, the percentage of polymorphic loci, the genetic diversity index and the number of lack loci of different populations were analysed and calculated with RAPD technique. The results indicated that a sample of more than 30 plants could represent the genetic characteristics of a population of 200 plants. Based on the principle that the size of propagating population should double the size of effective one in germplasm regeneration, a sample of no less than 60 plants should be appropriate for regenerating Fowering Chinese Cabbage germplasm.
出处
《植物遗传资源学报》
CAS
CSCD
2004年第1期43-46,共4页
Journal of Plant Genetic Resources
基金
农业部资源保护项目蔬菜种质资源的更新 (No .0 2 5 )