摘要
目的 研究大鼠IgE的Fc区CH2 3的生物学活性。方法 构建大鼠IgE的Fc区CH2 3的原核表达质粒pBAD gⅢA CH2 3,并转化入TOP10中 ,阿拉伯糖诱导表达、周质腔抽提、Ni NTA金属鳌合柱纯化获得大鼠IgE的Fc区CH2 3,细胞及动物水平检测蛋白质生物学活性。结果 原核系统表达出大鼠IgE的Fc区CH2 3,它能够阻断OVA激发的RBL 2H3的脱颗粒反应 ,阻断被动皮肤实验。结论 大鼠IgE的Fc区CH2 3能够封闭IgE高亲和力受体 。
Objective To study the biological activity of the rat IgE Fc C H2-3. Methods To transfer the pBAD/gⅢA/C H2-3 cons-tructed by us into E.Coil Top10. Rat IgE Fc C H2-3 was produced in periplasmic space of E.Coil Top10 by inducing it with arabinose.Biological activity was detected on cells and animals. Results Gene of rat IgE Fc C H2-3 was successfully amplified and cloned into pBAD/gⅢA. It was obtained by expression. It can inhibit passive sensitization of skin mast cells in vivo. It can also block the degranulation of sensitized RBL-2H3 by anti-OVA-IgE in vitro. Conclusions Rat IgE Fc C H2-3 can block IgE and high affinity receptor-Fc ε RⅠ, and inhibit the allergic reactions
出处
《免疫学杂志》
CAS
CSCD
北大核心
2004年第3期213-216,共4页
Immunological Journal
基金
默沙东哮喘基金 (2 0 0 0年 )
关键词
IGE
恒定区
原核表达
变态反应
IgE
Constant domain
Prokaryotic expression
Allergic reaction
