宫血宁对大鼠离体子宫的节律收缩作用

Effects of Gongxuening on phasic contractions in isolated Rat uterus

目的 探讨宫血宁治疗妇科出血的药理作用机制 ,为临床用药提供理论依据。方法 大鼠离体子宫张力实验法。结果 发现大鼠离体子宫给药前没有明显的收缩。加入宫血宁至终浓度为 2 5 μg·ml-1 时 ,可引起离体子宫的节律性收缩 ,随着宫血宁给药剂量的增加 ,离体子宫收缩幅度增大、频率加快。宫血宁 2 0 0 μg·ml-1 时的收缩强度可接近 0 .0 0 1u/ml缩宫素的作用。宫血宁给药后 4 0min内 ,子宫收缩幅度下降不明显 ,而收缩频率略有下降。其作用同缩宫素相似 ,而优于米索前列醇。结论 宫血宁能引起大鼠离体子宫的收缩作用 ,并具有明显的剂量效应。

Aim Gongxuening is traditional Chinese Materia Medica,which is used for therapy of uterine hemorrhage with various etiology.The pharmacological mechanism of Gongxuening on uterus hemorrhage were inuestigated.Methods Tension measurement of intact myometrial strips was used.Results It was found spontaneous activity of isolated rat uterus was not obvious and as little as 25μg·ml -1 Gongxuening could quickly induce uterine phasic contractions.With the increase of Gongxuening concentrations,both contractile amplitude and frequency were accelerated.The contractile activity of 200μg·ml -1 Gongxuening was similar to that of 0.001u/ml Oxytocin.There was no significant decreasing in contractile activity within 40min after administration of Gongxuening or Oxytocin,while contractile frequency was decreased obviously in misoprostol group.Conclusion Gongxuening could stimulate phasic contractions in isolated rat uterus in a dose-dependent manner.