反相高效液相色谱法测定人血浆中替米沙坦的浓度

Determination of telmisartan in human plasma by RP-HPLC

目的 建立RP -HPLC法测定人血浆中替米沙坦浓度。方法 用AgilentZORBAX×ODS柱 (4 .6× 1 5 0mm ,5 μm) ,流动相为水 -乙腈 -三乙胺 (6 0∶4 0∶0 .1 ) ,荧光检测激发波长为 30 0nm ,发射波长为 380nm ,采用萘乙酸为内标。结果 替米沙坦保留时间为 9min左右 ,浓度线性范围 1 0～ 35 0 0 μg·L-1 。结论 该法准确、快速、简便 。

Aim To establish a high performance liquid chromatography(RP-HPLC)method to determine the concentration of telmisartan in human plasma.Methods Separation was achieved on the Agilent ZORBAX×ODS column(4.6×150mm,5μm).The mobile phase consisted of water-Acetonitrile-Triethylamine(60∶40∶0.1,v/v/v) and was used at the flow rate of 1.0ml/min.The fluorimetric excitation and emission wavelengths were set at 300nm and 380nm,respectively.Naphtalene-1-acetic acid was used as internal standard.Results The retention time of telmisartan was about 9min.The calibration curves were linear in the range of 10～3 500μg·L -1 .Conclusion The HPLC method can be used to determine the concentration of telmisartan in human plasma.