高效液相色谱法测定蛇花颗粒中绿原酸的含量

Determination of chlorogenic acid in Shehua Keli by high performance liquid chromatography

目的 建立蛇花颗粒中绿原酸的含量测定方法。方法 采用高效液相色谱法 ,以ZORBAXSB -C1 8(4 .6mm× 2 5 0mm ,5 μm)为色谱柱 ;流动相 :乙腈 - 0 .4 %磷酸溶液 (1 3∶87) ;检测波长 :32 7nm ;柱温 :2 5℃ ;结果 绿原酸在 0 .0 4 4 2～ 0 .4 4 2 0 μg范围内 ,浓度与峰面积线性关系良好 (r=0 .9998) ;样品的平均加样回收率为 :99.36 % ,RSD 为 1 .36 % ,(n=6 )。结论 方法简便、快速 。

Aim To investigate a method for determination of chlorogenic acid in Shehua Keli.Methods In HPLC,ZORBAX SB-C 18 was used as the stationary phase,acetonitrile-0.4% phosphoric acid(13∶87) was used as mobile phase.The photodiode array detector was used at 327nm.Column temperature was 25℃.Results The calibration curve was linear over the range of 0.044 2～0.442 0μg(r=0.999 8).The average recovery of the method was 99.36%,RSD 1.36%(n=6).Conclusion The results showed that this method was reliable and accurate.The method could be applied to separate and determine the chlorogenic acid in Shehua Keli.