抗肝细胞生长多肽单克隆抗体及其抗独特型抗体的建立及初步应用

HYBRIDOMA AND MONOCLONAL ANTIBODY(McAb) FOR HEPATOCYTE STIMULATOR PROTEIN(HSP)

为了深入研究肝细胞生长因子及其与肝细胞作用的机制,本文应用单克隆抗体技术获得两株分泌肝细胞生长多肽单克隆抗体的杂交瘤细胞系:以纯化的两株单抗为抗原,我们制备了抗独特型兔血清抗体,发现它们具有肝细胞生长多肽样作用.本文应用单克隆抗体检测250例正常人和肝病病人血清(免疫斑点法),发现重型肝炎病人血清肝细胞生长多肽含量明显增高,肝性脑病病人更为明显;慢性迁延性肝炎与正常人比较无显著差异.提示血清肝细胞生长多肽升高与肝细胞损伤程度有关.4例肝癌病人3例血清肝细胞生长多肽阳性。

We have purified HSP from liver of human fetus by improved LaBrecque' S method, Immunized BALB/C mice with HSP,we adopted activity-inhibition experiment of HSP as remedy for ELISA method to screen useful hybredoma clone, Finally, we obtained 2 antibodyproducing clonies, which produced lgG3 and IgGl that revealed clearly the dose-dependent inhibitive activity for HSP, The antiidiotype antibodies of anti-HSP showed similiar stimulative activity as HSP, Determining serum HSP by the anti-HSP, we discovered higher level .in patients with fulminant hepatic failure