金灰藓(Pylaisiella polyantha)配子体发生的实验观察

Experimental Observations on the Gametophyte Development of Pylaisiella polyantha

金灰藓Pylaisiellapolyantha(Hedw.)Grout孢子在光照培养箱中进行培养,实验组为含有Knop培养液的琼脂培养基质,对照组为无营养成分的琼脂培养基质。在光学显微镜下对配子体发生进行了观察、描绘和照相。结果表明:实验组和对照组孢子萌发所需时间相同,均为53.5h,萌发极相为1～4极。4d时,实验组萌发率为85.4%,对照组为54.1%;20d时,开始形成配子体芽原基;40d时,形成具假根、茎、叶的配子体,并形成两性器官。并对配子体发生过程的特点进行了分析和讨论。

Spore germination and protonema development of Pylaisiella polyantha (Hedw.) Grout in response to Knop and distilled water. The medium of these two condition is agar. The spores of Pylaisiella polyantha is well developed, its protonemata and gametophyte are formed. The process from the spore germination and protonema growth to development of the gametophyte are illustrated and taken microphotographs. The result shows that the time of spore germination at two condition is 53.5 hours, the germination pole is 1-4 poles.There is a contrast to germination rate of the two groups. After 4 days, the germination rate of experiment group is 85.4%, the other is 54.1%. About the 20th day bud primordium appears. Nearly 40th day, the gametophyte fully grow and starts development of sexual organs. Besides, the characteristic about (deve-)(lopment) of the gametophyte are analysed and discussed in this paper.