湖北推广小麦品种(系)高分子量麦谷蛋白亚基组成分析

Analysis of High-molecular-weight Glutenin Subunit Compositions from Hubei Commercial Hexaploid Wheat (Triticum aestivum L.) Cultivars

应用SDS-PAGE技术分析了45份湖北推广小麦品种(系)籽粒的高分子量麦谷蛋白亚基组成。40份材料的高分子量麦谷蛋白亚基组成为同质,5份为异质。在Glu-1位点共检测到9种等位基因变异类型,其中Glu-A1位点有"1、2*、Null"3种变异类型,Glu-B1位点有"7、7+8、7+9、14+15"4种,Glu-D1位点有"2+12、5+10"2种。"Null、7+8、2+12"是主要亚基,它们的频率分别是62.5%、60%和72.5%。亚基组合类型有12种,其中(Null,7+8,2+12)亚基组合占30.0%,(1,7+8,2+12)、(1,14+15,2+12)、(Null,7+9,2+12)、(Null,7+8,5+10)4种组合的频率都在10%以上,这5种亚基组合占总组合的72.5%。供试小麦材料品质评分在5～10之间,平均评分为7.0。含5+10亚基的品种(系)所占比例低,是湖北小麦烘烤品质较差的部分原因。

Sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to determine the high-molecular-weight glutenin subunit (HMW-GS) composition of 45 hexaploid wheat (Triticum aestivum L.) cultivars from Hubei Province.Of which 40 cultivars are homogeneous for HMW-GS composition and 5 cultivars are heterogenous.Nine alleles have been identified at Glu-1 loci and which are three at Glu-A1 (1, 2~*, Null),four at Glu-B1 (7, 7+8, 7+9, 14+15) and two at Glu-D1 (2+12 and 5+10),respectively.The major HMW-GS at Glu-1 loci are Null,7+8, 2+12,with the frequencies of 62.5%, 60.0% and 72.5%,respectively.Twelve HMW-GS combinations have been observed,of which five combinations,(Null, 7+8, 2+12),(1, 7+8, 2+12),(1, 14+15, 2+12),(Null, 7+9, 2+12),(Null,7+8, 5+10) have accounted up to 72.5%,with each combination high than 10% in all and the first combination up to 30%.The Glu-1 quality scores of the tested cultivars range from 5 to 10 with an average score of 7.0 . The low frequency of subunits 5+10 implies that the analyzed cultivars having poor bread-making quality.