抗癌药物敏感试验(MTT法)在胃肠道恶性肿瘤化疗中的临床应用

ANTINEOPLASTIC DRUG SENSITIVITY TEST (MTT ASSAY) IN CHEMOTHERAPY OF GASTROINTESTINAL MALIGNANCY-A REPORT ON PRELIMINARY CLINICAL APPLICATION

作者采用抗癌药药敏试验(MTT法),对33例胃肠道癌(25例结直肠癌,8例胃癌)进行6种抗癌药药物敏感性测定.结果表明:除4例因取样不足,组织培养不理想或培养污染外,检6成测功获者7.%;5-Fu、MMC在其余23例结直肠癌体外药敏试验中,显示敏感者分别为8.7%和21.7%;6例胃癌为16.7%和33.3%,该测试系统进一步证明,瘤细胞对抗癌药的反应存在着明显个体差异.本法有快速、简便、重复性好的优点。

Thirty-three cases of gastrointestinal cancers, 25 colorectal and 8 gastric, were subjected to MTT assay using 6 antineoplastic drugs in vitro. Excluding 4 cases, 2 colorectal and 2 gastric, in which the assay failed because of insufficient sampling, unsatisfactory tissue culture or contamination of the cultures, the assay succeeded in 87.9% of cases in this group. The sensitive rates for 5-fluorouracil and mitomy-cin C in colorectal cancers were 8.7% and 21.7% and those in gastric cancers were 16.7% and 33.3%. Further studies showed that there existed significant individual differences in the response of tumor cells to different drugs. We concluded that MTT assay had the advantages of quick and simple manipulation and easy to repeat, and it was suggested that it should be popularized clinically in the Treatment of malignancy