华东地区PMWS患病猪群PCV-2的检测及其基因特征

Detection and Genetic Characterization of Porcine Circovirus(PCV-2) from Pigs with Postweaning Multisystemic Wasting Syndrome in Mid-east of China

用 PCR方法 ,对江苏、上海、山东、江西、安徽和浙江等省市 6 5家猪场 99例断奶仔猪多系统衰弱综合征(PMWS)肺脏和淋巴结病料 ,进行了猪圆环病毒 - 2 (PCV- 2 )基因检测 ,发现其中 18家猪场 2 1例病料为 PCV- 2阳性。源自安徽和上海的 PCV- 2分离株经基因序列分析显示 ,2个分离株部分 ORF2 与国外分离毒株同源性为 92 %～99%。安徽分离株 AHHF6与韩国分离株 KSY- 1同源性为 99% ,而上海分离株 SHHT1与加拿大分离株 Imp.114 7同源性为 98%。上述结果表明 ,我国 PMWS患病猪群广泛存在 PCV- 2 ,不同地区 PCV- 2存在一定基因差异 。

Ninety-nine pigs with postweaning multisystemic wasting syndrome from 65 pig farms in Jiangsu,Shanghai,Shandong,Jiangxi,Anhui and Zhejiang provine,were investigated to detect the type 2 porcine circovirus(PCV-2) in their lungs and lymph nodes.The results showed that 21 samples of 18 pig farms contained PCV-2 gene.To determine the extent of genetic heterogeneity of PCV-2 isolates,the partia ORF of two PCV-2 isolates from different province were amplified by PCR and sequenced.The results indicated that they had 92%-99% similarity with others isolated from other countries.The two isolates vary,to some extent,in their genomic sequence,AHHF6 isolate is closely related to the Korea isolate KSY-1,and SHHT1 isolate is similar to the Canadian isolate imp.1147.It showed that the viruses existed wildly in our country and might be original from different geographic regions.