放射型根瘤菌分批发酵生产辅酶Q_(10)的代谢特性和发酵动力学

Metabolic Characters and Fermentation Kinetics of Batch Cultures to Produce CoQ_(10) by Rhizobium radiobacter

以放射型根瘤菌WSH2601作为辅酶Q10分批发酵的试验菌,对其代谢特性进行了初步研究。对分批发酵过程中细胞生长、产物积累、糖消耗、中间产物有机酸及pH的变化规律进行了描述;由Logistic模型方程分别建立了放射型根瘤菌辅酶Q10发酵过程细胞生长、产物合成及基质消耗随时间变化的数学模型。模型模拟计算结果与实验值能较好地吻合。动力学研究结果表明该模型能较好地反映放射型根瘤菌的细胞生长、底物消耗和产物合成过程及其动力学机制。辅酶Q10分批发酵中细胞生长与产物合成属于半偶联型。

Preliminary study of the metabolic characters of CoQ10 batch fermentation with Rhizobium radiobacter WSH2601 was carried out. Cell growth, CoQ10 accumulation, sugar consumption, organic acids formation and pH changes throughout the CoQ10 fermentation were investigated. Mathematic models of cell growth, CoQ10 formation and substrate consumption in CoQ10 fermentation were proposed according to the Logistic model equation. Good coincidence between models calculated values and experimental data could be observed. The results of dynamics studies show that the model equations could really reflect the CoQ10 fermentation process and kinetic mechanism by Rhizobium radiobacter WSH2601. CoQ10 formation is partially growth associated in all batch cultures with Rhizobium radiobacter WSH2601.