丝裂素活化的蛋白激酶在双歧杆菌预防大肠癌中的作用

The effection of mitogen activated protein kanace on prevention of large bowel carcinoma with Bifidobacterium

目的 :从信号转导这一层次探索双歧杆菌预防大肠癌生长的机制。方法 :以大肠癌裸鼠移植瘤为动物模型 ,预先用青春型双歧杆菌注射于裸鼠腹腔 ,然后以激光共聚焦显微镜检测大肠癌移植瘤组织丝裂素活化的蛋白激酶 (MAPK)家系中的ERK1/ 2、JNK和p38的含量。结果 :双歧杆菌预防组大肠癌组织ERK1/ 2的平均荧光强度明显低于肿瘤对照组 (P <0 0 1) ,而JNK和p38的平均荧光强度在两组间差异无显著性 (P >0 0 5 )。结论 :青春型双歧杆菌通过抑制ERK1/ 2的活化来预防大肠癌的生长。

Objective:To investigate the mechanisms of Bifidobacteria to prevent the growth of lager bowel carcinoma in view of signal transduction.Methods:The animal model of large bowel carcionma nude mouse transplantation tumors was set up. Bifidobacteria adolescence were intraperitoneally injected into nude mice in advance.The content of ERK1/2、JNK and p38 of mitogen activated protein kinase(MAPK) family in large bowel carcinoma transplantation tumor tissues was detected by using laser scanning confocal microscopy.Results:The average fluorescent strength of ERK1/2 of large bowel carcinoma tissues in bifidobacterium prevention tissues was significantly lower when compared with the tumor control group( P >0 01) The average fluorescent strength of JNK and P38 was no difference in the two groups( P >0 05) Conclusion: Bifidobacteria adolescene could prevent the growth of large bowel carcinoma through inhibing the ERK1/2 activation.