人乳头瘤病毒16型L1和L2基因表达产物的鉴定

Immunocompetent study of expression of human papillonavirus type 16 capsid protein L1 and L2 in E.coli

目的 :构建人乳头瘤病毒 (humanpapillomavirus,HPV) 16型晚期基因L1及L2的原核表达质粒 ,并验证目的蛋白的表达。方法 :用限制性酶切及连接的方法构建原核表达质粒 pET3a 16L1和 pET3a 16L2 ,通过SDS PAGE及Westenblot检测目的融合蛋白的表达。结果 :在大肠菌中诱导表达的L1蛋白分子量约为 5 7KD ,L2蛋白分子量约为 90KD。结论 :该实验结果为HPV16型预防性基因工程亚单位疫苗的研制和诊断试剂的研究开发奠定了基础。

Objective:To construct prokaryotic expression vector of HPV type 16 L1 and L2 gene.Methods:L1 and L2 genes deriving from recombinant vector pUC18 L1 and pUC18 L2 were subcloned into the expression vector pET3a respectivly.Then,analysing the expression of aim protein though SDS PAGE and Western blot.Results:The MW of L1 protein is approximately 57 KD and that of the L2 protein is approximately 90 KD.Conclusion:These results will be the basis to develop the HPV16 prophylactic vaccines and diagnosis Kit.