马来酸麦角新碱化学发光传感器的研究

Flow Injection Chemiluminescence Sensor for the Determination of Ergonovine Maleate

基于发光试剂通过电价键将铁氰化钾固定在阴离子交换树脂上 ,可直接对待测物马来酸麦角新碱进行传感 ,从而建立测定药物马来酸麦角新碱的传感器新方法。其线性范围为 5× 10 -3 ～ 1mg/L ,线性方程为ΔI =10 6 .4 5C +17.10 (R2 =0 .998) ,检出限达到 2 .6 μg/L。对 0 .0 1和 0 .1mg/L的马来酸麦角新碱进行10次测定 ,它们的相对标准偏差 (RSD)分别为 2 .1%、2 .4 %。此传感器的稳定性良好 ,可连续使用 2 0 0次以上。

A novel flow injection chemiluminescence (CL) sensor for the determination of ergonovine maleate has been developed. Hexacyanoferrate(III) was immobilized electrostatically on an anion-exchange resin column and was eluted from the column using sodium phosphate solution as eluent. The sensor responds linearly to ergonovine maleate concentration in the range of 5.0 x 10(-3) to 1.0 mg /L with a detection limit ( 3sigma) of 2.6 mug/L. The sensor is stable for determinations 200 times and has been successfully applied to the determination of ergonovine maleate in injections and urine samples.