以单分散交联聚甲基丙烯酸环氧丙酯树脂为基质的亲水性排阻色谱固定相的合成及其在生物大分子分离中的应用

Preparation of Hydrophilic Size Exclusion Packings Using Monodisperse Poly(glycidylmethacrylate-co-ethylenedimethacrylate)Beads as Matrix and Their Application for Biopolymers Separation

以单分散交联聚甲基丙烯酸环氧丙酯树脂为基质 ,将该树脂经化学改性后得到亲水性良好的新型尺寸排阻色谱固定相 ,其表面羟基含量高达 6 .1mmol/ g。详细评价了该改性后树脂对蛋白的质量回收率、亲水性、耐压性能及化学稳定性。用三羟甲基胺基甲烷 (Tris)缓冲溶液 ( pH =7)为流动相 ,对蛋白质混合样的分离遵循体积排阻色谱分离机理。

Based on monodisperse poly ( glycidylmethacrylate-co-ethylenedimethacrylate) particles as a media, a new hydrophilic stationary phase for size exlusion chromatography (SEC) was synthesized by a new chemically modified method. The content of hydroxyl was measured to be 6.1 mmol/g. The modified resin was evaluated for mass recovery of proteins, hydrophilicity, rigidity, and chemical stability in detail. With trihydroxymethylaminomethane ( Tris) buffer ( pH = 7) as mobile phase, three kinds of proteins and a peptide were selected to test the synthesized SEC column. It was abserved that it follows SEC mechanism and a satisfactory result was obtained.