全二维气相色谱/飞行时间质谱用于莪术挥发油分离分析特性的研究

Analytical Characteristics of Zedoary Volatile Oil by Comprehensive Two-dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

用全二维气相色谱 /飞行时间质谱 (GC×GC/TOFMS)研究莪术挥发油。对GC×GC与GC的分离特性和GC×GC/TOFMS与GC/MS的定性能力进行了比较。在相同条件下 ,GC分离出 87个峰 ,GC×GC分出约 5 0 0个峰 ,GC/MS和GC×GC/TOFMS鉴定出匹配度大于 80 0的组分分别为 4 6种和 2 2 7种。除此之外 ,GC×GC/TOFMS对每一个组分可给出三维定性信息 ,定性可靠性大大提高。研究结果显示与传统的分析技术相比 。

The comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC x GC/TOFMS) was applied to investigate zedoary volatile oil. The separation power of GC x GC and GC, and identification power of GC x GC/TOFMS and GC/MS were compared under the same conditions. 87 peaks were achieved with GC, while about 500 peaks were resolved with GC x GC. In the meantime, GC x GC/TOFMS not only identified 227 peaks (much more than the peaks of 46 in GC), but also provided 3 kinds of orthogonal identification information that makes the result more reliable. All of these show that GC x GC/TOFMS is a powerful instrument in the analysis of volatile oil of traditional Chinese medicines.