针刺对偏头痛大鼠血浆神经肽的影响

Effects of acupuncture on plasma neuropeptide in migraine rats

目的:探讨针刺治疗偏头痛对降钙素基因相关肽(calatoningene-ralatedpeptide,CGRP)、P物质、内皮素、神经肽Y、心钠素、神经降压肽(neurotensin,NT)的影响。方法:将40只SD大鼠数字表法随机分为正常对照组、模型对照组、针刺对照组和针刺治疗组。按各组方案造模,或针刺治疗后,采用放射免疫法测定颈静脉血浆中多种神经肽的含量。结果:模型对照组CGRP(319.64±53.12)ng/L;P物质(126.76±39.29)ng/L;心钠素(366.11±83.12)ng/L;内皮素(785.00±186.64)ng/L;神经肽Y(4567.06±1041.86)ng/L均较正常对照组(152.87±31.82),(57.43±21.03),(278.31±58.76),(516.88±126.89),(3232.75±424.87)ng/L有明显升高(t=2.283～2.791,P<0.05或0.01),而模型对照组NT(56.64±21.21)ng/L较正常对照组(113.12±35.92)ng/L明显降低(t=2.842,P<0.01);针刺治疗组CGRP(148.54±19.18)ng/L;P物质(60.76±22.31)ng/L;心钠素(287.63±68.64)ng/L;内皮素(524.38±122.55)ng/L;神经肽Y(3324.56±576.77)ng/L均明显下降(t=2.833～2.481,P<0.05或P<0.01),NT(101.13±27.69)ng/L较模型对照组明显升高(t=2.669,P<0.05)。结论:针刺调节脑神经肽的合成与释放,是其治疗偏头痛的主要因素之一。

AIM:To explore the effects of acupuncture on plasma calcitonin gene-related p eptide(CGRP), substance P(SP),endothelin(ET),neuropeptide Y (NPY),atrial natriur etic factor atrial natriuretic polypeptide(ANP) and neurotensin(NT) in migraine rats. METHODS:Forty SD rats were randomly allocated into normal control group,model control group, acupuncture control group and acupuncture treatment group.After m odeling or acupuncture,levels of plasma neuropeptides in external jugular vein(E JV) in each group were determined with radioimmunoasay method. RESULTS:Rats in the model group had higher levels of CGRP[(319.64±53.12)ng/L] ,SP[(126.76±39.29)ng/L], ANP[(366.11±83.12)ng/L],ET[(785.00±186.64) ng/L]and NPY[(4 567.06±1 041.8) ng/L]as compared with the normal control group[CGRP(152. 87±31.82) ng/L;SP(57.43±21.03) ng/L;ANP(278.31±58.76) ng/L;ET(516.88±126. 89) ng/L;NPY(3 232.75±424.87) ng/L](t=2.283-2.791,P< 0.05 or P< 0.01),but the levels of NT in the model control group was decreased significantly to(56.64 ±21.21) ng/L,as compared with that in the normal control group[(113.12±35.92) ng/L,t=2.842,P< 0.01].Compared with the model control group,the level of CGRP,SP ,ANP,ET and NPY were significantly decreased to (148.54±19.18),(60.76±22.31),( 287.63±68.64),(524.38±122.55) and(3 324.56±576.77) ng/L in the acupuncture tr eatment group(t=2.833-2.481,P< 0.05 or P< 0.01),while the level of NT was sig nificantly increased to(101.13±27.69) ng/L(t=2.669,P< 0.05). CONCLUSION:Acupuncture can regulate the synthesis and release of neuropeptides , which is one of the main factors in the treatment of migraine.