海风藤选择性抑制淀粉样前体蛋白基因表达(英文)

Selective inhibition of haifengteng in gene expression of beta-amyloid precursor protein

背景:淀粉样蛋白(β-amyloidprotein,β-AP)是老年斑的核心成分,它在脑内的沉积与阿尔茨海默病(Alzheimer'sAD)发病有着密切的关系。现代研究证实β-AP来源于淀粉样前体蛋白(β-amyloidprecursorprotein,β-APP),如果β-APP基因表达受到抑制,β-AP在脑内的沉积就会减少,阿尔茨海默病(Alzheimerdisease,AD)有可能得到有效防治。因此,很多国内外学者都在积极寻找β-APP基因表达抑制物,企图发现防治AD的有效药物。目的:研究海风藤对人类神经母细胞瘤细胞β-APP基因表达的影响。设计:药物(海风藤)干扰和非药物干扰对照;目的基因(β-APP)与结构基因(β-Actin)对照。材料:人类神经母细胞瘤细胞系列(SK-N-SH),海风藤。方法:应用细胞培养和反转录—聚合酶链反应(RT-PCR)观察海风藤不同浓度和不同时间对β-APPmRNA的影响。结果:海风藤选择性的抑制β-APP基因表达,这种抑制作用随时间的延长和浓度的增加而增强。结论:海风藤选择性抑制β-APP基因表达,为其防治AD提供了更加可靠的实验依据。

BACKGROUND:Beta-amyloid protein(β-AP) is a core element in senile plague an d its cerebral deposition closely relates to the onset of Alzheimer's disease(AD ).Modern researches prove that β-AP is originated form beta-amyloid precursor protein(β-APP).If the gene expression of β-APP can be inhibited,the cerebra l deposition of β-AP will decrease,and therefore,AD might be treated effective ly.Hence,many national and international scholars are actively searching for the inhibitors that could inhibit the gene expression of β-APP for effective medi cations in AD treatment. OBJECTIVE:To study the impact of haifengteng on gene expression of β-APP in the cells of human neuroblastoma. DESIGN:Experimental control: medication intervention(haifengteng) and non-med ication intervention.Gene comparison between objective gene(β-APP) and structu re gene(β-Actin). MATERIALS:Cellular serials of human neuroblastoma(SK-N-SH), haifengteng. METHODS:To observe the impact of haifengteng in different reactive strength an d different reactive time on β-APP mRNA through cellular culture and RT-PCR. RESULTS:Haifengteng could selectively inhibit the gene expression of β-APP a nd the inhibition enhanced along with the prolongation of the reactive time and the increase of the reactive strength. CONCLUSION:The selective inhibition of haifengteng in gene expression of β-A PP provides a more reliable laboratorial basis in AD prevention and treatment.