再生障碍性贫血瘦素水平的测定

The study on the leptin level of aplastic anemia

目的 :通过对再生障碍性贫血 (再障 )患者体内瘦素水平的测定 ,了解再障骨髓造血微环境异常与瘦素的关系。方法 :按正常人、再障、其他类型贫血分为三组 ,每位受试者均抽取骨髓液、外周血 ,用放射免疫分析法分别测定其瘦素含量。结果 :再障组骨髓液和外周血瘦素水平分别与正常人及其他类型贫血组比较 ,差异均有统计学意义 (P <0 .0 1) ,其他类型贫血组与正常人比较 ,差异无统计学意义 (P >0 .0 5 ) ,但三组的骨髓液与外周血瘦素含量差异均有统计学意义 (P <0 .0 1) ,但三组相关系数差异无统计学意义 (P >0 .0 5 )。结论 :再障患者骨髓液、外周血瘦素含量明显高于正常人及其他类型贫血 ,可能与再障骨髓脂肪细胞明显增多 ,脂肪细胞分泌瘦素增多有关。瘦素受体的不完整及造血干 /祖细胞对干细胞因子 (SCF)的反应性降低 ,破坏了瘦素与SCF协同刺激原始造血祖细胞增殖的作用 ,可能是造成再障瘦素水平高 ,而造血干 /祖细胞数量减少的一方面原因。

Objective: To study the leptin level in patients with aplastic anemia, and explore the relationship between leptin and hematopoietic microenviroment abnormality of bone marrow under aplastic anemia.Method:Radioimmunoassay (RIA) was used to measure leptin level of bone marrow and peripheral blood from normal persons, patients with aplastic anemia and patients with other types anemia, respectively.Result:The leptin level of bone marrow and peripheral blood had high significant difference (P< 0.01) between alpastic anemia groups and healthy controls or other types anemia groups, while no significant difference(P> 0.05) was found between the two latter. For three groups, leptin level of bone marrow was significantly correlated with that of peripheral blood, and the correlation coefficient has no significant difference (P> 0.05).Conclusion:The leptin level of bone marrow and peripheral blood from patients with aplastic anemia was signifiantly higher than that from normal persons or patients with other types anemia, which may be related to the remarkable increase of adipocytes in bone marrow of aplastic anemia. Incomplete of leptin receptors and low responsiveness of hematopoietic stem/progenitor cells to stem cell factor may destroy the synergy stimulation effects of leptin and stem cell factor on proliferation of primitive hematopoietic progenitors, which may be one of the reasons of why leptin level of aplastic anemia is high while the quantity of hematopoietic stem/progenitor cell number is low.