摘要
以欧洲七叶树幼嫩叶片为外植体 ,在离体条件下成功诱导出体细胞胚胎 (体胚 )。研究结果表明 ,诱导愈伤组织的适宜培养基是MS +2 ,4 -D 2mg/L +KT 0 2mg/L ,MS +BA 8mg/L +NAA 1mg/L有利于胚性愈伤的诱导和增殖 ,添加ZT 2mg/L或BA 5mg/L和NAA 0 2mg/L的MS培养基有利于体胚发育和成熟。
Somatic embryogenesis systems were investigated by means of vitro culture from young leaf of Aesculus hippocastanum L.The results showed that embryogenic calli were induced from young leaves,which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +NAA 0.2mg/L.
出处
《分子植物育种》
CAS
CSCD
2003年第5期649-654,共6页
Molecular Plant Breeding
基金
上海市农委科技兴农科技计划资助。
