半日花的组织培养及植株再生(简报)

TISSUE CULTURE AND PLANT REGENERATION OF HELIANTHEMUM SONGARICUM SCHRENK

半日花(Helianthemum Songaricum Schrenk)是半日花科半日花属落叶小灌木,分布于新疆、甘肃和内蒙古等荒漠地区海拔1000米至1300米山地.

The cotyledonary segments of sterile seedlings of Helianthemum Songaricum Schrenk were cultured on different media containing different phytohormones. It was found that the calli could be induced efficiently on MS basal medium supplemented with 10. 0mg/L NAA and 0. 2mg/L 6-BA . When calli were transferred on MS medium with 2. 0mg/L 6-BA and 0. 2mg/L NAA, shoots were produced. The frequency of shoot differentiation reached about 85%. The regenerated shoots were rooted on 1 /2MS medium added with 0. 5mg/L NAA. The rooting rate was about 76% . Regenerated plantlets were successfully transplanted in soil, with a success rate of 67% .