摘要
目的 建立血中乙醇的测定方法 ,研究饮酒后乙醇在血中的代谢状况 ,调查酒后驾车肇事者血中乙醇的浓度水平 ,探索简便、快速检测血中乙醇的实用方法。方法 采受试者手指末梢血 ,处理后直接进样 ,气相色谱法分析。对饮酒量与不同代谢时间的血中乙醇浓度进行相关性分析 ,对酒后肇事驾驶员血中乙醇浓度水平进行统计分析。结果 本文建立的方法 ,血中乙醇的最小检出浓度为 0 .0 0 5mg/ml。当标准溶液在0 .0 75 8～ 1 .5 6 1mg/ml浓度范围内 ,相对标准偏差为 4 .2 5 %～ 2 .89% ;实验数据表明 ,饮酒量与血中乙醇浓度显著相关 (R2 =0 .9732 )。调查结果显示 ,大于 80 % (共 1 0 5例 )酒后肇事驾驶员血中乙醇超过 89.9mg/dl。结论 血中乙醇直接进样气相色谱分析法 ,简便、准确 ,具有实用价值。驾驶员血中乙醇含量接近 90mg/dl时 ,行为能力受影响 。
Objective To establish a gas chromatography method for detecting ethanol in blood and research metabolic mechanism of ethanol and investigate ethanol level in blood after drinking alcohol that trouble drivers. Methods Sampling blood from finger twig and analyzed by straight inject GC. Statistical analysis of relationship of ethanol concentration level in blood and time-response of drivers after drinking alcohol was made. Results When ethanol concentration were 0.0758～1.561 mg/ml, the relative standard deviation (RSD) was 4.25%～2.89%. The detection limit of blood ethanol was 0.005 mg/ml. Experiment data indicated that there was significant correlation of drinking alcohol amount and blood ethanol concentration (R 2 =0.9732). Investigation founded that blood ethanol concentration more than 89.9 mg/dl for 80% samples of 150 troubled drivers after drinking alcohol.
出处
《安徽预防医学杂志》
2004年第3期142-144,共3页
Anhui Journal of Preventive Medicine
关键词
饮水
血中乙醇
酒后肇事
blood ethanol
gas chromatography
drinking alcohol
