天然生物材料壳聚糖支架上人胚肺成纤维细胞的生长

Growth of Human Fetal Lung Fibroblasts on the Natural Biomaterial-Chitosan Scaffold

采用不同粒度的硅胶粒子作为致孔剂 ,按硅胶和壳聚糖重量比 9∶1,制备了三组不同孔径的壳聚糖多孔支架 .以无孔壳聚糖支架为参照 ,对多孔支架的有效孔径、吸水性进行了比较 .结果表明 :孔径大小由硅胶尺寸控制 ,吸水性随孔径增大而增大 .为研究支架孔径大小对其生物相容性的影响 ,在系列支架上进行了人胚肺成纤维细胞的培养 .细胞种植 1d后 ,多孔支架上的细胞粘附较多 ,而无孔支架上的细胞伸展情况较好 ;细胞培养 5d后 ,所有支架上细胞伸展情况良好 ,孔径越大的支架上细胞增殖越多 .

A series of porous chitosan scaffolds were fabricated with various sizes of silica particles as porogen at chitosan/silica weight ratio of 9:1. The effective pore sizes and bibulous ability of the porous scaffolds were compared with those of non-porous films. It shows that the pore size is determined by the size of silica particles while the bibulous ratio is enhanced with increasing pore sizes. To evaluate their biocompatibility, human fetal lung fibroblasts, were cultured on these scaffolds. The results show that all the scaffolds availed to the cell growth. After one day of cell seeding, the porous scaffolds held more viable cells than the nonporous did, while cells spread better on the control film. Five days later, the cell proliferation occurred on all the scaffolds, whereas considerably more cells proliferated on the porous ones, and the bigger the pore size was, the faster cell proliferation was achieved. These results would contribute, to the application of chitosan scaffolds in tissue engineering.