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Type Two Cytokines Predominance of Human Lung Cancer and Its Reverse by Traditional Chinese Medicine TTMP 被引量:11

Type Two Cytokines Predominance of Human Lung Cancer and Its Reverse by Traditional Chinese Medicine TTMP
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摘要 Type 2 cytokines are usually predominant in tumor patients and associated with tumor progression.To explore whether reversing of type 2 predominance could be a promising strategy in tumor immunotherapy,PBMCs of 35 lung cancer patients and 19 healthy subjects were prepared and subjected to be examined for cytokine secretion and gene expression.Tetra-Methylpyrazine (TTMP),extracted from a traditional Chinese medicinal herb which has been used in clinic to reverse the Th2 status of cancer patients in China,was added to PBMC culture. Determined by RT-PCR,the positive percentages of mRNA expression of type 1 cytokines (8.6% for IFN-γ and 11.4% for IL-2) were lower than those of type 2 cytokines (71.4% for IL-4,60% for IL-6 and 80% for 1L-10) in patients' PBMCs.The potential of gene expressing (measured as relative intensity to the ratio of β-actin) in the patients for type 1 cytokines was also in a low level (0.111 for IFN-γ,0.119 for IL-2) in comparison with a relative high level for type 2 cytokines (0.319 for IL-4,0.303 for IL-6 and 0.377 for IL-10).Meanwhile,both positive percentage and relative intensity of gene expression were lower for a type 1 cytokine-related transcription factor T-bet (31.4% and 0.142,respectively) than those for type 2 cytokine-related GATA3 (85.7% and 0.378, respectively).The blood serum levels of IFN-γ and IL-2 in the patients were slightly lower but not significantly when compared with healthy control.In contrast,the levels IL-4 and IL-6 in patients were significantly higher than those in healthy subjects by ELISA analysis.TTMP could enhance supernatant concentration and gene expression levels of IFN-γ,IL-2 and T-bet,but reduced those of type 2 cytokines.These results demonstrate that the lung cancer patients had a predominant expression of type 2 cytokines and TTMP could reverse the type 2 dominant status,which might offer an alternative therapeutic regime for lung cancer patients.Cellular & Molecular Immunology.2004;1(1):63-70. Type 2 cytokines are usually predominant in tumor patients and associated with tumor progression.To explore whether reversing of type 2 predominance could be a promising strategy in tumor immunotherapy,PBMCs of 35 lung cancer patients and 19 healthy subjects were prepared and subjected to be examined for cytokine secretion and gene expression.Tetra-Methylpyrazine (TTMP),extracted from a traditional Chinese medicinal herb which has been used in clinic to reverse the Th2 status of cancer patients in China,was added to PBMC culture. Determined by RT-PCR,the positive percentages of mRNA expression of type 1 cytokines (8.6% for IFN-γ and 11.4% for IL-2) were lower than those of type 2 cytokines (71.4% for IL-4,60% for IL-6 and 80% for 1L-10) in patients' PBMCs.The potential of gene expressing (measured as relative intensity to the ratio of β-actin) in the patients for type 1 cytokines was also in a low level (0.111 for IFN-γ,0.119 for IL-2) in comparison with a relative high level for type 2 cytokines (0.319 for IL-4,0.303 for IL-6 and 0.377 for IL-10).Meanwhile,both positive percentage and relative intensity of gene expression were lower for a type 1 cytokine-related transcription factor T-bet (31.4% and 0.142,respectively) than those for type 2 cytokine-related GATA3 (85.7% and 0.378, respectively).The blood serum levels of IFN-γ and IL-2 in the patients were slightly lower but not significantly when compared with healthy control.In contrast,the levels IL-4 and IL-6 in patients were significantly higher than those in healthy subjects by ELISA analysis.TTMP could enhance supernatant concentration and gene expression levels of IFN-γ,IL-2 and T-bet,but reduced those of type 2 cytokines.These results demonstrate that the lung cancer patients had a predominant expression of type 2 cytokines and TTMP could reverse the type 2 dominant status,which might offer an alternative therapeutic regime for lung cancer patients.Cellular & Molecular Immunology.2004;1(1):63-70.
出处 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2004年第1期63-70,共8页 中国免疫学杂志(英文版)
基金 supported partly by 0utstanding Young Scientist Award(#30125038) Key Project(#30230340)by Natural Science Foundation of China Chinese Key Basic Science Program by Ministry of Science and Technology of China(#2001CB510009) Foundation of Chinese Academy of Science(#KSCX2-2-08) UICC Yamagiwa-Yoshida Memorial International Cancer Study Grant.
关键词 Th2 cytokine predominance lung cancer Chinese medicine Tetra-Methylpyrazine Th2 cytokine predominance lung cancer Chinese medicine Tetra-Methylpyrazine
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参考文献15

  • 1Edmund C. So,Kar-Lok Wong,Tian-Chyuan Huang,Sheng-Chou Tasi,Chi-Feng Liu PhD.Tetramethylpyrazine protects mice against thioacetamide-induced acute hepatotoxicity[J].Journal of Biomedical Science.2002(5)
  • 2Chi-Feng Liu,Mei-Hsiu Lin,Chun-Ching Lin,Hui-Wen Chang,Prof. Song-Chow Lin PhD.Protective effect of tetramethylpyrazine on absolute ethanol-induced renal toxicity in mice[J].Journal of Biomedical Science.2002(4)
  • 3Norimasa Ito,Hiroshige Nakamura,Hiroyuki Metsugi,Shigetsugu Ohgi.Dissociation Between T Helper Type 1 and Type 2 Differentiation and Cytokine Production in Tumor-Infiltrating Lymphocytes in Patients with Lung Cancer[J].Surgery Today.2001(5)
  • 4Mark Boothby,Ana L. Mora,Mark A. Aronica,Jeehee Youn,James R. Sheller,Shreevrat Goenka,Linda Stephenson.IL-4 signaling, gene transcription regulation, and the control of effector T cells[J].Immunologic Research (-).2001(2-3)
  • 5Jun Feng,Ruiyuan Liu,Guozhen Wu,Shuben Tang.Effects of tetramethylpyrazine on the release of PGI2 and TXA2 in the hypoxic isolated rat heart[J].Molecular and Cellular Biochemistry (-).1997(1-2)
  • 6Sudo N,Yu XN,Aiba Y,et al.An oral introduction of intestinal bacteria prevents the development of a long-term Th2-skewed immunological memory induced by neonatal antibiotic treatment in mice[].Clinical and Experimental Allergy.2002
  • 7Bicalho HMS,Gontijo M,Nogneira-mahado JA.A simple technique for simultaneous human leukocytes separation[].Journal of Immunological Methods.1981
  • 8Dobashi K,Aihara M,Araki T,et al.Regulation of LPS induced IL-12 production by IFN-gamma and IL-4 through intracellular glutathione status in human alveolar macrophages[].Clinical and Experimental Immunology.2001
  • 9Lee HJ,Takemoto N,Kurata H,et al.GATA3 reduces T helper cell type 2(Th2) cytokine expression and chromatin remodeling in committed Th1 cells[].The Journal of Experimental Medicine.2000
  • 10Wu CC,Chiou WF,Yen MH.A possible mechanism of action of tetramethyl-pyrazine on vascular smooth muscle in rat aorta[].European Journal of Pharmacology.1989

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