NF-κBp65在豚鼠内耳的表达与分布

Expression of NF-κB p65 in the inner ear of guinea pigs

目的 :观察NF -κBp6 5 (nuclearfactor -kappaBp6 5 ,NF -κBp6 5 )在正常豚鼠内耳的表达与分布。方法 :健康杂色豚鼠 6只 ,取耳蜗和内淋巴囊组织 ,石蜡包埋切片 ,用大鼠NF -κBp6 5单克隆抗体行免疫组织化学 (SABC法 )常规染色 ,以正常豚鼠肾脏组织作阳性对照。结果 :在正常豚鼠内耳中 ,NF -κBp6 5主要表达于螺旋韧带和内淋巴囊及其周围组织。细胞内定位主要在胞浆内 ,仅个别细胞胞核有NF -κBp6 5表达。结论 :螺旋韧带和内淋巴囊在正常情况下含有较多的NF -κB ,提示螺旋韧带和内淋巴囊重要的功能之一是产生和分泌细胞因子 ,它们可能是调控内耳炎性反应的“中枢”部位。

Objective:To investigate the expression of NF-κB p65 in the inner ear of guinea pigs.Method: The endolymphytic sacs(ES) and cochlea of six healthy pigmented guinea pigs were embedded in paraffin and serial sections were mounted onto slides. The sections were routinely stained with immunohistochemistry by mouse NF-κB p65 monoclonal antibody. The kidney tissues of guinea pigs were used as positive controls. Result: The preponderance of immunostaining for NF-κB p65 were observed in the spiral ligaments(SL), ES and perisaccular region. Localization of NF-κB p65 in the above mentioned cells was mainly in the cytoplasm and occasionally in the nucle.Conclusion:Abundant NF-κB are found within the SL and perisaccular region and that suggest producing inflammatory cytokines may be part of their normal function. The SL and perisaccular region may be the 'central sites' of controlling inflammatory cellular response in the inner ear.