茄子体细胞杂种游离小孢子培养获得再生植株

Plantlet Regeneration by Isolated Microspore Culture of Somatic Hybrid of Eggplant

应用游离小孢子培养技术,培养茄子体细胞融合杂种DSa不同株系的小孢子。结果表明:对花药进行3C℃高温6 d预处理,能显著提高小孢子脱分化能力;小孢子离体培养的最佳发育时期是单核期;小孢子培养的适宜培养基为KM附加PEG 250 mg/L、2,4-D 0.2 mg/L、ZT 0.5 mg/L、NAA 1mg/L及6.5％的葡萄糖;再生植株适宜培养基为MS附加ZT 2 mg/L、IAA 0.1 mg/L、2％蔗糖和7g/L琼脂。

Somatic fusion hybrid DSa (S. torvum Sw x S. melongena L. ' Dourga' ) microspore were cultured by using isolated microspore culture. The result show that microspore dedifferentiation ability was markedly improved by using the treatment of anther under the high temperature conditions of 36℃ for continuous 6 days. The best development period for microspore culture is monokaryotic stage; The suitable medium for microspore culture is KM with PEG 250 mg/L, 2, 4-D 0. 2 mg/L, ZT 0. 5 mg/L, NAA 1 mg/L and glucose 6.5%; The suitable medium for regenerated plantlet is MS with ZT 2 mg/L, IAA 0. 1 mg/L, sugar 2% and agar 7 g/L.